Syllabus for Entrance test
for Admission to M Sc Biotechnology Course
Note: The syllabus prescribed
for the entrance test has been divided into fifteen units. Each unit carries a
weightage of four marks. Paper setters are required to set four multiple choice
type questions with only one correct or most appropriate answer separately for
each unit, giving uniform representation to the whole syllabus contained
therein.
UNIT I
General science: Structure and
properties of water. Ion product of water. Buffers, pH, pK and pI. Basic
concepts on Molarity, Molality and Normality. Mean, Mode, Median, Standard
deviation, Standard error and probability. Basics of computer sciences and its
applications to biology.
UNIT II
Bio-Energetic & Biological
Interaction: Laws of Thermodynamics .Concepts of heat of reaction, enthalpy,
and entropy. Concept of chemical bonding, Electron displacements, Dipole-dipole
interactions, hydrogen bond, Vander-Waal’s forces, hydrophobic and hydrophilic
Interactions, Acid-base equilibria, Soaps and detergents.
UNIT III
Bio-molecules: Carbohydrates,
Lipids, Proteins, Vitamins and nucleic acids-Types, structure and function.
UNIT IV
Cell biology: Prokaryotic and
eukaryotic cell-structure and organelle functions. Functions of sub-organells
of cell i.e., Nucleus, Endoplasmic reticulum, Golgi apparatus, Peroxisomes and
mitochondria. Structure of Cell membrane types and regulation of transport.
UNIT V
Metabolism: Carbohydrate,
Lipids, amino acids and nucleotides turn over and its regulation, Metabolic
disorders. Glycolysis, TCA cycle, Urea cycle, Gluconeogenesis, Glyoxalate cycle
and pentose phosphate pathway.
UNIT VI
Biological oxidations:
Oxidation-reduction potentials, electron acceptors and donors in plants and
animals. ATP
synthesis-oxidative and photo-phosphorylation.
UNIT VII
Molecular Biology: Prokaryotic
and eukaryotic Replication, Transcription Translation, mechanism and their
regulation. DNA Repair systems. Recombination.
UNIT VIII
Concept of Recombinant DNA
Technology : Cloning vectors: Plasmids, Bacteriophages, Cosmids,
phagemids, YACs, Restriction
enzymes, Ligases, PCR.
UNIT IX
Immunology: Innate and adaptive
immunity, Antigen and super antigens. Structure and functions of
immunoglobulin. T-cells and
B-cells.
UNIT X
Bio-Techniques: Principles,
types and applications of Chromatography, Centrifugation, Electrophoresis,
Spectrophotometry and Blotting techniques.
UNIT XI
Genetics: Mendelian laws of
inheritance and their application, linkage and crossing over, gene mapping,
theories of mutation and evolution, Genetic disorders.
UNIT XII
Animal & Plant physiology:
Physiology and development of circulatory, nervous system. Endocrine
and exocrine system: hormone
diversity and action. Transport across plant cell, Transpiration, Photosystems,
Flowering, plant hormones. Plant tissue culture and production of transgenic
plants
UNIT XIII
Microbiology: Structure and
organization of microbial cells, Microbial growth, Transformation,
Transduction, Conjugation.
Antimicrobial agents. Drug resistance
UNIT XIV
Enzymology: History, general
characteristics, nomenclature and classifications of enzymes, enzyme
activity and factors affecting
enzyme activity, Competitive and uncompetitive inhibition, allosteric
enzymes.
UNIT XV
Cancer biology: Cell cycle
progression and regulation. Receptor mediated cell Signaling. Different
types of receptors,
Secondary messengers. Protein modification with specific emphasis on
oncogenesis. Protein modification by phosphorylation and de-phosphorylation and
the role in signal transduction processes.